FRR Culture Collection Catalogue
Media
Carnation Leaf Agar (CLA)
Czapek Yeast Extract Agar (CYA)
Czapek Yeast Extract Agar with 20% Sucrose (CY20S)
Dichloran Chloramphenicol Malt Agar (DCMA)
Malt Extract Agar (MEA)
Malt Extract Yeast Extract 50% Glucose Agar (MY50G)
Malt Extract Yeast Extract 5% Salt 12% Glucose Agar (MY5-12)
Potato Dextrose Agar (PDA)
Tap Water Agar (TWA)
Carnation Leaf Agar (CLA)
Add several sterile dried carnation leaf pieces, preferably gamma-irradiated,
to Petri dishes just prior to addition of 10 to 15 mL of TWA.
Czapek Yeast Extract Agar (CYA)
| K2HPO4 | 1.0g |
| NaNO3 | 3.0 g |
| MgSO4.7H2O | 0.5 g |
| KCl | 0.5 g |
| FeSO4.7H2O | 0.01 g |
| Sucrose | 30.0 g |
| Yeast extract | 5.0 g |
| Agar | 15.0 g |
| Water, distilled | 1.0 L |
Add 1.0 mL/L of trace metals solution. Sterilise by autoclaving at 121°C for 15
minutes.
(Trace metals solution: 1% w/v ZnSO4.7H2O and 0.5% w/v
CuSO4.5H2O in distilled water).
Czapek
Yeast Extract Agar with 20% Sucrose (CY20S)
| K2HPO4 | 1.0 g |
| NaNO3 | 3.0 g |
| MgSO4.7H2O | 0.5 g |
| KCl | 0.5 g |
| FeSO4.7H2O | 0.01 g |
| Sucrose | 200.0 g |
| Yeast extract | 5.0 g |
| Agar | 15.0 g |
| Water, distilled | 1.0 L |
Sterilise by autoclaving at 121°C for 15 minutes.
Dichloran Chloramphenicol Malt Agar (DCMA)
| Malt extract | 10 g |
| Dichloran (0.2% w/v in 95% ethanol) | .0 mL |
| Chloramphenicol | 0.1 g |
| Agar | 15.0 g |
| Water, distilled | to 1.0 L |
Sterilise by autoclaving at 121°C for 15 minutes.
| Malt extract (powdered) | 20.0 g |
| Glucose | 20.0 g |
| Peptone | 1.0 g |
| Agar | 20.0 g |
| Water, distilled | 1.0 L |
Sterilise by autoclaving at 121°C for 15 minutes.
Malt Extract Yeast Extract 50% Glucose Agar (MY50G)
| Malt extract (powdered) | 10.0 g |
| Yeast extract | 2.5 g |
| Agar | 10.0 g |
| Water, distilled | 500.0 g |
| Glucose A.R. | 500.0 g |
Add minor constituents and agar to 500.0 g distilled water and steam to
dissolve agar. Add glucose rapidly while stirring. Sterilise by steaming for 30
minutes.
Do not autoclave.
Malt
Extract Yeast Extract 5% Salt 12% Glucose Agar (MY5-12)
| Malt extract (powdered) | 20.0 g |
| Yeast extract | 5.0 g |
| NaCl | 50.0 g |
| Glucose | 120.0 g |
| Agar | 20.0 g |
| Water, distilled | to 1.0 L |
Sterilise by autoclaving at 121°C for 10 minutes.
| Potatoes | 250.0 g |
| Glucose | 20.0 g |
| Agar | 15.0 g |
| Water, distilled | 1.0 L |
Dice unpeeled potatoes into 500 mL of water, then steam or boil for 30 to 45 minutes.
Melt agar in 500 mL of water. Strain potato through several layers of cheese cloth into
a flask containing the melted agar. Squeeze some potato pulp through the cheese cloth
as well. Add the glucose and mix thoroughly, make up to 1 L with distilled water. Sterilise
by autoclaving at 121°C for 15 minutes.
| Agar | 20.0 g |
| Water, distilled | 1.0 L |
Sterilise by autoclaving at 121°C for 15 minutes.